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C3997 Sigma

Anti-CAPZB (71-85) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-CAPB, Anti-CAPPB, Anti-CAPZ, Anti-F-actin-capping protein subunit β isoform 2

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Properties

Related Categories Alphabetical Index, Antibodies, CAM-CB, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~31 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CAPZB(832)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 71-85 of human CAPZB

General description

The gene CAPZB (F-actin-capping protein subunit β) is mapped to human chromosome 1p36.1. The protein co-localizes with the actin filaments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-CAPZB (71-85) antibody produced in rabbit is suitable for western blot at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

CAPZB (F-actin-capping protein subunit β) are F-actin-capping proteins. Absence of CAPZB reduces actin filament depolymerization and capping. CAPZB also plays a role in the regulation of cell morphology. T cell surface protein CD2 regulates T cell signalling partly by associating with CAPZB, CD2AP (CD2-associated protein) and CIN85 (SH3 domain-containing kinase-binding protein 1).

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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