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C3998 Sigma

Anti-COL13A1 (251-265) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-COLXIIIA1, Anti-Collagen α-1(XIII) chain isoform 1

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Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~70 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL13A1(1305)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 251-265 of human COL13A1

Antigen Background

COL13A1 (251-265) encodes the alpha chain of one of the nonfibrillar collagens. The function of this gene product is not known, however, it has been detected at low levels in all connective tissue-producing cells so it may serve a general function in conn

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-COL13A1 (251-265) antibody is suitable for western blot at a dilution of 1:500-1:2,000. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

COL13A1 is involved in cell-matrix and cell-cell adhesion interactions, necessary for normal development. It also binds heparin.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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