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  • C4231 - Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal

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C4231 Sigma

Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal

clone BC-22, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells, Antibodies for Pluripotent Stem Cells,
species reactivity   chicken, rat, mouse, human
application(s)   indirect ELISA: suitable
  microarray: suitable
  western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132
clone   BC-22, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
mol wt   antigen mol wt ~94 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphos­phorylated peptide. It does not detect the unphos­phorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.

Application

Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for
• indirect ELISA
• microarray
• western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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