|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells, Antibodies for Pluripotent Stem Cells,|
|species reactivity||chicken, rat, human, mouse|
|application(s)||indirect ELISA: suitable|
|western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|mol wt||antigen mol wt ~94 kDa|
|shipped in||dry ice|
human ... CTNNB1(1499)|
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphosphorylated peptide. It does not detect the unphosphorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
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