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C4481 Sigma

Anti-Caspase 4 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
species reactivity   human
application(s)   immunoprecipitation: 5-10 μg using 300 μg lysate of human ECV304 endothelial cells.
  microarray: suitable
  western blot (chemiluminescent): 1:250 using whole cell extract of human ECV304 endotheloid cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 43-47 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP4(837)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to human procaspase 4α isoform sequence, (amino acids 81-98) with N-terminal lysine added, conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Application

Anti-Caspase 4 antibody produced in rabbit is suitable for:
• immunoprecipitation at a concentration of 5-10μg using 300μg lysate of human ECV304 endothelial cells.
• microarray
• western blotting (chemiluminescent)at a dilution of 1:250 using whole cell extract of human ECV304 endotheloid cells

Biochem/physiol Actions

Caspase-4 is a 377 amino acids protein "upstream" caspase having a long N-terminal prodomain. Caspase-4 is capable of cleaving itself and the p30 caspase-1 precursor, and of inducing apoptosis in transfected insect or COS cells.

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