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  • C4562 - Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse

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C4562 Sigma

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse

clone hHCD, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Actin-associated Proteins/Myosin,
species reactivity   bovine, sheep, mouse, rabbit, pig, human
application(s)   immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
  immunoprecipitation: suitable
  western blot: 1:4,000 using human uterus extract
clone   hHCD, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CALD1(800)
mouse ... Cald1(109624)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human uterus smooth muscle extract.

Specificity

The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture.

Application

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for:
• immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
• immunoprecipitation
• western blot at a dilution of 1:4,000 using human uterus extract

Biochem/physiol Actions

Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon).

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