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C4710 Sigma

Monoclonal Anti-Cyclin A antibody produced in mouse

clone CY-A1, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cyclins and CDKs,
species reactivity   human, bovine, monkey, mouse
application(s)   immunocytochemistry: suitable
  microarray: suitable
  western blot: 1:1,000 using a mouse NIH 3T3 cell extract
clone   CY-A1, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 60 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CCNA2(890)
mouse ... Ccna2(12428)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant bovine cyclin A.

General description

Cyclin A is a nuclear protein during S phase and it binds both cdk2 and cdc2. The availability of monoclonal antibody reacting specifically with cyclin A enables the subcellular detection and localization of cyclin A and the measurement of relative differences in cyclin A levels as a function of cell cycle phase.

Specificity

The antibody recognizes an epitope present in amino acids 162-433 of bovine cyclin A. No cross-reactivity is detected with other cyclins (cyclin B, D, or E).

Application

Monoclonal Anti-Cyclin A antibody produced in mouse is suitable for:
• immunocytochemistry
• microarray
• western blotting at a dilution of 1:1,000 using a mouse NIH 3T3 cell extract

Price and Availability


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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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