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C4731 Sigma

Anti-Calnexin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers and Receptors, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling,
species reactivity   human, mouse, rat, canine
application(s)   immunoprecipitation: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line
  indirect immunofluorescence: 1:200 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line
  microarray: suitable
  western blot: 1:2,000 using whole cell RIPA lysate of the human hepatocytoma HepG2 cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 90 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CANX(821)
mouse ... Canx(12330)
rat ... Canx(29144)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to the c-terminus of human calnexin (amino acids 573-592) conjugated to KLH.

General description

Calnexin, is a calcium binding type I integral membrane protein present mainly in the endoplasmic reticulum. It plays a pivotal role in quality control processes during protein synthesis and folding. Anti-calnexin antibody (diluted 1: 300) can be used in dual immunofluorescence staining to visualize TG2 transport. Rabbit anti-calnexin antibody reacts specifically with calnexin (90 kDa) of dog, mouse, rat and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-calnexin antibody can be used in immunoblotting and immunoprecipitation. It can also be used in microarray and western blotting.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Peer-Reviewed Papers
15

References

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Description

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C7617 Anti-Calnexin antibody, Mouse monoclonal, clone TO-5, purified from hybridoma cell culture
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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