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C4851 Sigma

Anti-Caspase 1 (ICE) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ICE

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
species reactivity   rat, human, mouse
application(s)   immunocytochemistry: 10 μg/mL using human HL-60 cells
  western blot: 0.5-2 μg/mL using human HL-60 cell lysate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 20 kDa (p20 subunit)
  antigen mol wt 45 kDa (p45 proenzyme)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP1(834)
mouse ... Casp1(12362)
rat ... Casp1(25166)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acid residues 129-152 of human caspase 1 conjugated to KLH.

Physical form

Solution of antiserum in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M sodium chloride, and 0.05% sodium azide.

Specificity

Recognizes the p20 subunit of reduced human caspase 1 isoforms and the p45 proenzyme.

Application

Anti-Caspase 1 (ICE) antibody produced in rabbit has been used in immunoblot assay to analyse CASP-3 and -7 cleavage of WT and DKO cells treated with etoposide. It is suitable for immunocytochemistry at a concentration of 10μg/mL using human HL-60 cells. It is also suitable for western blot at a concentration of 0.5-2μg/mL using human HL-60 cell lysate.

Biochem/physiol Actions

ICE1 is a MYC-like basic helix-loop-helix transcription factor. It is an upstream activator of the cold-induced transcriptome. ICE1 belongs to the bHLH subfamily 9. It specifically binds to the MYC recognition sites (5′-CANNTG-3′) on the CBF3/DREB1A promoter. ICE1 regulates the cold-induced transcription of CBF/DREB1 genes.

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