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C4859 Sigma

Cycloheximide solution

Ready-Made Solution, microbial, 100 mg/mL in DMSO, 0.2 μm filtered

Synonym: CHX

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Description

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Specificity

Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.

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Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2810 6.1 / PGII
WGK Germany 
3
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Peer-Reviewed Papers

References

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Cycloheximide-induced cPLA(2) activation is via the MKP-1 down-regulation and ERK activation. Lin, W.W., and Hsu, Y.W. Cell. Signal. 12, 457, (2000)

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A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models. Dai X, James RG, Habib T, et al. J. Clin. Invest. 123(5), 2024-36, (2013)

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Activation and control of p53 tetramerization in individual living cells. Gaglia G, Guan Y, Shah JV, et al. Proc. Natl. Acad. Sci. U. S. A. 110(38), 15497-501, (2013)

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NMDA receptor subunits in the adult rat hippocampus undergo similar changes after 5 minutes in an open field and after LTP induction. Baez MV, Oberholzer MV, Cercato MC, et al. PLoS ONE 8(2), e55244, (2013)

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Synthesis and evaluation of antifungal properties of a series of the novel 2-amino-5-oxo-4-phenyl-5,6,7,8-tetrahydroquinoline-3-carbonitrile and its analogues. Atul R Gholap et al Bioorg. Med. Chem. 15, 6705-15, (2007)

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Small molecules with structural similarities to siderophores as novel antimicrobials against Mycobacterium tuberculosis and Yersinia pestis. Karen L Stirrett et al Bioorg. Med. Chem. Lett. 18, 2662-8, (2008)

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The protein level of PGC-1α, a key metabolic regulator, is controlled by NADH-NQO1. Adamovich Y, Shlomai A, Tsvetkov P, et al. Mol. Cell. Biol. 33(13), 2603-13, (2013)

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Decrease of WNK4 ubiquitination by disease-causing mutations of KLHL3 through different molecular mechanisms. Mori Y, Wakabayashi M, Mori T, et al. Biochem. Biophys. Res. Commun. 439(1), 30-4, (2013)

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HSV carrying WT REST establishes latency but reactivates only if the synthesis of REST is suppressed. Zhou G, Du T, and Roizman B Proc. Natl. Acad. Sci. U. S. A. 110(6), E498-506, (2013)

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Antifungal thiopeptide cyclothiazomycin B1 exhibits growth inhibition accompanying morphological changes via binding to fungal cell wall chitin. Naoko Mizuhara et al Bioorg. Med. Chem. 19, 5300-10, (2011)

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Effect of antimicrobial compounds on Balamuthia mandrillaris encystment and human brain microvascular endothelial cell cytopathogenicity. Ruqaiyyah Siddiqui et al Antimicrob. Agents Chemother. 51, 4471-3, (2007)

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Synthesis of somatostatin analogues containing C-terminal adamantane and their antiproliferative properties. Anna Miyazaki et al J. Med. Chem. 51, 5121-4, (2008)

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In vitro cytotoxicity of melleolide antibiotics: structural and mechanistic aspects. Markus Bohnert et al Bioorg. Med. Chem. Lett. 21, 2003-6, (2011)

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The ubiquitin-proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic β-cells. Hofmeister-Brix A, Lenzen S, and Baltrusch S Biochem. J. 456(2), 173-84, (2013)

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Synthesis of sugars embodying conjugated carbonyl systems and related triazole derivatives from carboxymethyl glycoside lactones. Evaluation of their antimicrobial activity and toxicity. Nuno M Xavier et al Bioorg. Med. Chem. 19, 926-38, (2011)

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Yeast glycogen synthase kinase-3beta pathway inhibitors from an organic extract of Streptomyces sp. Sarot Cheenpracha et al J. Nat. Prod. 72, 1520-3, (2009)

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Matrix metalloproteinase-2 (MMP-2) generates soluble HLA-G1 by cell surface proteolytic shedding. Rizzo R, Trentini A, Bortolotti D, et al. Mol. Cell Biochem. 381(1-2), 243-55, (2013)

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Genomewide screening for genes associated with gliotoxin resistance and sensitivity in Saccharomyces cerevisiae. Georgios Chamilos et al Antimicrob. Agents Chemother. 52, 1325-9, (2008)

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Analysis of physico-chemical properties of substrates of ABC and MFS multidrug transporters of pathogenic Candida albicans. Nidhi Puri et al Eur. J. Med. Chem. 45, 4813-26, (2010)

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Hyperosmolarity-induced up-regulation of claudin-4 mediated by NADPH oxidase-dependent H2O2 production and Sp1/c-Jun cooperation. Ikari A, Atomi K, Yamazaki Y, et al. Biochim. Biophys. Acta 1833(12), 2617-27, (2013)

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Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation. Neukamm SS, Ott J, Dammeier S, et al. J. Biol. Chem. 288(23), 16403-15, (2013)

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A role for thioredoxin-interacting protein (Txnip) in cellular creatine homeostasis. Zervou S, Ray T, Sahgal N, et al. Am. J. Physiol. Endocrinol. Metab. 305(2), E263-70, (2013)

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A structural analysis of the differential cytotoxicity of chemicals in the NCI-60 cancer cell lines. Suman K Chakravarti et al Bioorg. Med. Chem. 16, 4052-63, (2008)

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Changes in translation rate modulate stress-induced damage of diverse proteins. Kim H and Strange K Am. J. Physiol. Cell Physiol. 305(12), C1257-64, (2013)

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New cyclic somatostatin analogues containing a pyrazinone ring: importance of Tyr for antiproliferative activity. Anna Miyazaki et al Bioorg. Med. Chem. Lett. 18, 6199-201, (2008)

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Salicylic acid resistance is conferred by a novel YRR1 mutation in Saccharomyces cerevisiae. Kodo N, Matsuda T, Doi S, et al. Biochem. Biophys. Res. Commun. 434(1), 42-7, (2013)

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Identification of a PTC-containing DlRan transcript and its differential expression during somatic embryogenesis in Dimocarpus longan. Fang ZZ, Lai CC, Zhang YL, et al. Gene 529(1), 37-44, (2013)

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Newcastle disease virus Malaysian strain AF2240 induces apoptosis in MCF-7 human breast carcinoma cells at an early stage of the virus life cycle. Ghrici M, El Zowalaty M, Omar AR, et al. Int. J. Mol. Med. 31(3), 525-32, (2013)

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Lower gastric secretion and higher incorporation of orotic acid into liver RNA in rats treated with 5-azacytidine and cycloheximide. Cihák A, Korková L, Kohout J, et al. Neoplasma 25(3), 317-27, (1978)

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