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C4879 Sigma

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Mass Spectrometry, Molecular Biology,
type   Type II
form   essentially salt-free, lyophilized powder
activity   ≥40 units/mg solid
mol wt   mol wt 25,656 Da by calculation
purified by   6× crystallization
solubility   1 mM HCl: soluble10 mg/mL, clear, colorless
foreign activity   α-chymotrypsin ≤1 unit/mg (prior to activation by trypsin)
storage temp.   −20°C
Gene Information   cow ... CTRB1(618826)

Description

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges and requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form α, β, γ , δ, and π chymotrypsin (depending upon the conditions of activation). Its pI is 8.97.

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5 g in poly bottle

100, 250 mg in poly bottle

Unit Definition

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Application

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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