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C4963 Sigma

Catalase−polyethylene glycol

lyophilized powder, ~40,000 units/mg protein

Synonym: PEG-Catalase

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Therapeutic Enzymes
form   lyophilized powder
mol wt   PEG mol wt 5,000
composition   Protein, ~50% E405
extent of labeling   ~40 mol PEG per mol protein
matrix attachment   secondary amine linkage.
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Catalase from bovine liver coupled to methoxy-polyethylene glycol.

Packaging

Package size based on protein content

Physical form

Contains PEG plus 5% citrate buffer salts

Application

Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.

Biochem/physiol Actions

Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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