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C4963 Sigma

Catalase−polyethylene glycol

lyophilized powder, ~40,000 units/mg protein

Synonym: PEG-Catalase

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Therapeutic Enzymes
form   lyophilized powder
mol wt   PEG mol wt 5,000
composition   Protein, ~50% E405
extent of labeling   ~40 mol PEG per mol protein
matrix attachment   secondary amine linkage.
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Catalase from bovine liver coupled to methoxy-polyethylene glycol.

Packaging

Package size based on protein content

Physical form

Contains PEG plus 5% citrate buffer salts

Application

Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.

Biochem/physiol Actions

Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What can I use to solubilize Catalase-polyethylene glycol, Product C4963?
It is soluble in water at a concentration of 1 mg/mL.
What is the specific activity of Catalase-polyethylene glycol, Product C4963?
The certificate of analysis on our website will have lot-specific activity information.
What is the solution stability of Catalase-polyethylene glycol, Product C4963?
We generally recommend preparing fresh solutions of Catalase-PEG. If stock solutions must be stored, we recommend first dissolving in water, then diluteing with an equal volume of glycerol. This 50% glycerol solution may be kept in the freezer at -20°C. Aqueous solutions held at 37°C were found to lose approximately half of the catalase activity after about 10 days.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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