C50120 Sigma

CM Sephadex® C-50

Synonym: CM Sephadex®, Carboxymethyl Sephadex®



Related Categories Analytical/Chromatography, Cation Exchange Media, Ion Exchange Chromatography, Molecular Biology, Protein Chromatography,
bead size   40-125 μm (dry)
pore size   ~200,000 Da exclusion limit
operating pH   6 - 10
capacity   4.0-5.0 meq/g ion exchange capacity
compatibility   mode of use weak cation exchange chromatography


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


10, 50, 100 g in poly bottle


Sephadex C-50 is used for cation exchange media, separation media, resins, ion exchange chromatography, protein chromatography and affinity chromatography. Sephadex® C-50 has been used to study biochemical characteristics of cobra venom and effects of vitamins on lactoperoxidase enzyme activity.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What molecular weight range is CM Sephadex® C-50, Product C50120, suitable for?
Sephadex C-50 is suitable for larger biomolecules such as proteins in the molecular weight range of 30 to 100 kDa.
How do I prepare CM Sephadex® C-50, Product C50120?
Weigh out the required amount of dry powder and suspend it in binding buffer. In general, 1 gram of dry powder CM Sephadex C-50 will give about 30 mL final volume, when swollen in saline buffer.  Swelling should be done at the pH at which the resin is going to be used. Complete swelling takes 1-2 days at room temperature, or 2 hours at 100°C over boiling water.
How do I regenerate CM Sephadex® C-50, Product C50120?
Depending on the nature of the sample, regeneration is normally preformed by washing with a high ionic strength buffer (1-2 M NaCl) and/or increasing pH, followed by re-equilibrating in binding buffer.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Pharmaceutical, Purification, Separation

Peer-Reviewed Papers


Related Products

replaced by

Product #



Add to Cart

GE17-0220-01 CM-Sephadex® C-50, GE Healthcare, 17-0220-01, pack of 100 g C50120-100G is replaced by GE17-0220-01

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