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  • C5118 - Monoclonal Anti-Cytochrome P450 1A2 antibody produced in mouse

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C5118 Sigma

Monoclonal Anti-Cytochrome P450 1A2 antibody produced in mouse

~2 mg/mL, clone 3B8C1, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CP12, Anti-CYP1A2, Anti-P3-450, Anti-P450(PA)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Multi-Drug Resistance, Antibodies to Other MDR-related Proteins,
species reactivity   human, rat
application(s)   immunohistochemistry: suitable
  western blot: 0.1-0.2 μg/mL using recombinant human cytochrome P450 1A2
clone   3B8C1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   mol wt ~55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CYP1A2(1544)
rat ... Cyp1a2(24297)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rat cytochrome P450 proteins.

Antigen Background

Cytochrome P450 1A2 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes specifically human and rat cytochrome P450 1A2 and does not cross-react with cytochrome P450 1A1 or 1B1.

Application

Monoclonal Anti-Cytochrome P450 1A2 antibody produced in mouse is suitable for:
• immunohistochemistry
• western blotting at a concentration of 0.1-0.2μg/mL using recombinant human cytochrome P450 1A2

Biochem/physiol Actions

Cytochrome P450 enzymes are a super family of heme containing mono-oxygenases that in humans are involved with oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body.

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Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Phase I Drug Metabolism

Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues...
Robert Gates
Biofiles Volume 6 Article 1
Keywords: Catalysis, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gastrointestinal, Gene expression, Immunohistochemistry, Metabolism, Metabolites, Oxidations, Post translational modifications, Reductions, Support, Western blot

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Peer-Reviewed Papers
15

References

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