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  • C5176 - Monoclonal Anti-Cytokeratin Peptide 4 antibody produced in mouse

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C5176 Sigma

Monoclonal Anti-Cytokeratin Peptide 4 antibody produced in mouse

clone 6B10, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   goat, hamster, guinea pig, feline, sheep, human, rabbit, canine
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue
  microarray: suitable
  western blot: suitable
clone   6B10, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 59 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... KRT4(3851)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytokeratin from human esophagus.

General description

Cytokeratins are intermediary filaments that are expressed during epithelial development. Cytokeratin-4 is largely found in non-epidermal, stratified epithelial tissues such as larynx, pharynx, vagina, exocervix and epiglottis among several others . Cytokeratin-4 has been implicated in Streptococcus agalactiae infection and genetic mutations in Cytokeratin-4 have been linked to white sponge naevus . Monoclonal Anti-Cytokeratin Peptide 4 antibody is specific for cytokeratin 4 in goats, hamsters, guinea pigs, cats, sheep, humans, rabbits, and dogs. The antibody weakly stains ciliated pseudostratified epithelium of bronchi. Ducts of skin sweat glands and prostate are focally stained by the antibody. Some endocervical columnar cells may also react with the antibody. This antibody is reactive with methanol or acetone fixed frozen sections, and with protease-digested formalin fixed paraffin embedded human tissues. Embedded methacarn fixed material can also be used for cytokeratin 4 demonstration.

Specificity

The antibody reacts with non-cornifying squamous epithelium (e.g., exocervix, esophagus and cornea). Ciliated pseudostratified epithelium of bronchi is weakly stained. Ducts of sweat glands and prostate are focally stained. The antibody may react with some endocervical columnar cells.

Application

Monoclonal Anti-Cytokeratin Peptide 4 antibody is suitable for use in a broad range of immunoassays such as immunohistochemistry (frozen or formalin-fixed, paraffin-embedded sections), immunoblot, dot blot, and indirect immunofluorescence (1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue). The antibody may also be used for western blot and microarray.

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