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C5275 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture

Synonym: Con A

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Concanavalin A (Con A), Glycan Recognizing Proteins,
sterility   aseptically processed
type   Type IV-S
product line   BioReagent
form   lyophilized powder
impurities   salt (Contains buffer salts and NaCl)
solubility   PBS: soluble5 mg/mL, slightly hazy
  H2O: soluble, slightly hazy
suitability   suitable for cell culture
storage temp.   −20°C

Description

Analysis Note

A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
GK6890000
Protocols & Articles
Peer-Reviewed Papers
15

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