• USA Home
  • C5421 - Cholesterol Oxidase from microorganisms

EMAIL THIS PAGE TO A FRIEND
C5421 Sigma

Cholesterol Oxidase from microorganisms

aqueous solution, ≥30 units/mg protein (biuret)

Synonym: Cholesterol:oxygen oxidoreductase

Purchase

Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
form   aqueous solution
solubility   50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
shipped in   dry ice
storage temp.   −70°C

Description

Preparation Note

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use.

Unit Definition

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

Application

Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites.

Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 °C. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of Cholesterol Oxidase

To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase.
Keywords: Enzyme activity, Extinction coefficient, Isomerizations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?