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C5467 Sigma

EX-CELL® ACF CHO Medium

Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: CHO Medium

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Properties

Related Categories CHO Media and Medium Optimization Kits, CHO Platform, Cell Culture, Serum-free Media
Quality Level   GMP
description   for research or for further manufacturing use
sterility   sterile-filtered
form   liquid
quality   Drug/Device Master File available
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

Other Notes

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

Reconstitution

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water
Protocols & Articles

Articles

Effects of Inhibiting Two Cell Cycle Modulating micrornas

Upper panels: Cells were plated 24 hours after LNA electroporation for the in situ IgG Secretion Assay4. Detection was performed 48 hrs after electroporation. High IgG secretion cutoff is 90th percen...
Nan Lin1, Ken Heuermann,2, Jessica Schlueter,2, Carol Kreader, Scott Knight,2 and Kevin Kayser1
1. Cell Engineering, SAFC, Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103, U.S.A. 2. Research Biotechnology, Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103, U.S.A.
Keywords: Gene expression, Transduction

HTST Treatment

In pharmaceutical manufacturing, viral contamination can pose a significant risk to patient safety and damage a company’s reputation. Processes that reduce the risk of viral contamination, such as Hi...
Aaron Mack, Daiva Dailide, Chas Hernandez, Erik Klahn, and Bruce Lehr
Sigma-Aldrich Corporation, Cell Sciences and Development, 2909 Laclede Ave., St. Louis, MO, 63103
Keywords: Amino acid analysis, Cell culture, Culture media, Degradations, Elemental analysis, High performance liquid chromatography, Inductively coupled plasma, Infrared spectroscopy, Mass spectrometry, Pharmaceutical, Precipitation, Reductions, Spectroscopy, Titrations, Vitamins

Identification of the Putative Rosa26 Locus

The biopharmaceutical industry has expressed considerable interest in targeted integration in CHO cells for therapeutic r-protein production applications. In previous studies we have used ZFN’s for s...
Scott Bahr, Nan Lin, Trissa Borgschulte, Jeanne Brooks, Henry George and Kevin Kayser
Cell Sciences and Development, SAFC/Sigma-Aldrich, St Louis, MO 63103 USA
Keywords: Cloning, Gene expression, Polymerase chain reaction, Sequencing

Improving Product Safety Profiles

Post-translational modifications have been shown to affect the bioactivity, clearance rates, immunogenicity and safety profiles of therapeutic glycoproteins. For example anti- N-glycolylneuraminic ac...
Mascarenhas, J., Achtien, K., Richardson, S., Sealover, N., Kaiser, J., Borgschulte, T., George, H., Kayser, K. and Lin, N
Cell Sciences and Development, SAFC Sigma Aldrich2909 Laclede Avenue, Saint Louis, MO 63103, USA
Keywords: Cell culture, Clinical, Eliminations, Gene expression, Glycosylations, High performance liquid chromatography, Mass spectrometry, Sequencing, Size-exclusion chromatography

MSX Amplification

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992). However, most Chinese Hamster Ovary (CHO) c...
Kate Achtien, Trissa Borgschulte, Nan Lin, Henry George, Kevin J. Kayser
Cell Sciences and Development, SAFC, Sigma-Aldrich, 2909 Laclede Avenue, Saint Louis, MO 63103, USA
Keywords: Amplification, Cloning, Gene expression, Indicators, Polymerase chain reaction, Polymerase chain reaction - quantitative

Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1...
Nan Lin, Dustin Davis, Natalie R. Sealover, Joaquina Mascarenhas, Henry J. George and Kevin J. Kayser
Cell Sciences and Development, SAFC/Sigma-Aldrich 2909 Laclede Ave., Saint Louis, MO 63103. U.S.A.
Keywords: Affinity chromatography, Chromatography, Gene expression, Polymerase chain reaction, Protein extraction

Overexpression of Serpinb1

We report the discovery and validation of a novel CHO cell engineering target (Serpinb1) that has significant effects in enhancing recombinant IgG productivity. We performed transcriptomic studies us...
Nan Lin, Jeanne Brooks, Natalie Sealover, Christopher Limmex, Henry J. George and Kevin J. Kayser
Cell Sciences and Development, SAFC/Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103 USA
Keywords: Gene expression, High performance liquid chromatography, Inflammation, Microarray Analysis, Transcription, Transduction, Transfection, cDNA microarrays

Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identified species-specific housekeeping genes (HKGs) for Chinese HamsterOvary (CHO) cells using data from microarray gene expression profiling. HKGs suitable for quanti...
Scott M. Bahr, Trissa Borgschulte, Kevin J. Kayser, Matthew V. Caple and Nan Lin
Cell Sciences and Development, SAFC Biosciences 2909 Laclede Avenue, Saint Louis, MO 63103, USA
Keywords: Amplification, Cell culture, DNA microarrays, Gas chromatography, Gene expression, Melting, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction, Transcription

Signal Peptide Optimization

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins. Signal peptides are known to have a strong impact on both the efficiency of protein secretion and c...
Joaquina Mascarenhas, Dustin Davis, Pegah Jalili, Trissa Borgschulte, Kevin Ray, Henry J. George, Kevin J. Kayser and Nan Lin
Cell Sciences and Development, SAFC/Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103. U.S.A.
Keywords: Atomic absorption spectroscopy, Cell culture, Gene expression, High performance liquid chromatography, Mass spectrometry, Size-exclusion chromatography, Transfection

Zinc Finger Nuclease Efficiency

Zinc Finger Nuclease (ZFN) technology has provided researchers with a tool for integrating exogenous sequences into most cell lines or genomes in a precise manner. Using current methods, the efficien...
Scott Bahr, Laura Cortner, Sara Ladley, Trissa Borgschulte, CHOZN® Platform Development Team
SAFC/Sigma-Aldrich, St Louis, MO 63103 USA, scott.bahr@sial.com
Keywords: Gene expression, Recombination, Sequencing

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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