Synonym: Collagen Type 4
|Related Categories||2D Cell Culture, Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors,|
|impurities||HIV and hepatitis B, none detected|
|HIV, hepatitis B and hepatitis C, none detected (PCR)|
|solubility||acetic acid: soluble0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)|
|suitability||suitable for cell culture|
|Gene Information||human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)|
Epithelial cells, endothelial cells, muscle cells, nerve cells
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Type IV collagen is highly expressed in chorionic villi cytotrophoblast cells. The same cells are also capable of expressing the cleaving enzyme, 70S type IV collagenase.1
Prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers.
Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.
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Bornstein and Traub Type I, solid, BioReagent, suitable for cell culture
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Bornstein and Traub Type IV, powder
Bornstein and Traub Type IV, powder
Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture
Certificate of Analysis
Certificate of Origin
A1) We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of...
BioFiles 2008, 3.8, 5.
Keywords: Centrifugation, Dialysis, Filtration, Purification, Sterilizations
Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis
Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation
1. Simultaneous expression of 70 kilodalton type IV collagenase and type IV collagen alpha 1 (IV) chain genes by cells of early human placenta and gestational endometrium Autio-Harmainen, H., et al. Lab. Invest., (1992) 67, 191-200
Bornstein, P. and Traub, W. The Proteins, (1979) 4, 411-605
Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. Xu H Matrix Biol. 30(1), 16-26, (2011)
Fabrication of seamless electrospun collagen/PLGA conduits whose walls comprise highly longitudinal aligned nanofibers for nerve regeneration. Ouyang Y, Huang C, Zhu Y, et al. J. Biomed. Nanotechnol. 9(6), 931-43, (2013)
Response surface methodology and Taguchi approach to assess the combined effect of formulation factors on minocycline delivery from collagen sponges. Ghica MV, Albu MG, Popa L, et al. Pharmazie 68(5), 340-8, (2013)
Electrospun collagen mimicking the reconstituted extracellular matrix improves osteoblastic differentiation onto titanium surfaces. Iafiscol M, Quirici N, Foltran I, et al. J. Nanosci. Nanotechnol. 13(7), 4720-6, (2013)
Electrospun collagen/poly(L-lactic acid-co-epsilon-caprolactone) hybrid nanofibrous membranes combining with sandwich construction model for cartilage tissue engineering. He X, Fu W, Feng B, et al. J. Nanosci. Nanotechnol. 13(6), 3818-25, (2013)
Visian toric ICL implantation for residual refractive errors after ICRS implantation and corneal collagen cross-linking in keratoconus. Jarade E, Dirani A, Fadlallah A, et al. J. Refract. Surg. 29(7), 444, (2013)
Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force. Wolf K, Te Lindert M, Krause M, et al. J. Cell Biol. 201(7), 1069-84, (2013)
Effect of Allium sativum and fish collagen on the proteolytic and angiotensin-I converting enzyme-inhibitory activities in cheese and yogurt. Shori AB, Baba AS, and Keow JN Pak. J. Biol. Sci. 15(24), 1160-7, (2012)
Hydrolysis of extracellular ATP by ectonucleoside triphosphate diphosphohydrolase (ENTPD) establishes the set point for fibrotic activity of cardiac fibroblasts. Lu D and Insel PA J. Biol. Chem. 288(26), 19040-9, (2013)
A comparison of two dental implant systems in partially edentulous patients: 4-month post-loading results from a pragmatic multicentre randomised controlled trial. Esposito M, Blasone R, Favaretto G, et al. Eur. J. Oral Implantol. 6(2), 169-79, (2013)
1-stage versus 2-stage lateral maxillary sinus lift procedures: 4-month post-loading results of a multicenter randomised controlled trial. Felice P, Pistilli R, Piattelli M, et al. Eur. J. Oral Implantol. 6(2), 153-65, (2013)
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