C5533 Sigma

Collagen from human placenta

Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

Synonym: Collagen Type 4

Properties

Related Categories 2D Cell Culture, Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors,
product line   BioReagent
form   powder
impurities   HIV, hepatitis B and hepatitis C, none detected (PCR)
solubility   acetic acid: soluble0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
suitability   suitable for cell culture
storage temp.   −20°C
Gene Information   human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Description

Application

Epithelial cells, endothelial cells, muscle cells, nerve cells

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Type IV collagen is highly expressed in chorionic villi cytotrophoblast cells. The same cells are also capable of expressing the cleaving enzyme, 70S type IV collagenase.1

Preparation Note

Prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al

Biochem/physiol Actions

Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers.

Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.

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