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C5896 Sigma

Choline Oxidase from Alcaligenes sp.

lyophilized powder, ≥10 units/mg solid

Synonym: Choline: oxygen 1-oxidoreductase

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Description

Packaging

100, 500, 1000 units in poly bottle

50 units in glass bottle

Unit Definition

One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.

Application

Choline oxidase from Alcaligenes sp. has been used in a study to investigate a choline biosensor constructed with chitinous membrane and its application in measuring cholinesterase inhibitory activities. Choline oxidase from Alcaligenes sp. has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.

Choline oxidase from Alcaligenes sp. has been used to evaluate choline biosensors constructed with chitinous membranes. The enzyme has also been used to analyse the photoreaction of choline oxidase with covalently bound flavin, using spectroscopic techniques. Furthermore, the product can be used for cholinesterase activity and phospholipid determination assays.

Biochem/physiol Actions

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as the intermediate and molecular oxygen as th eprimary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the study of the reaction mechanism for the conversion of choline to the aldehyde intermediate, & of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approx. 72,000 Da (gel filtration) or 66,000 Da (SDS gel electrophoresis).

Physical properties

Isoelectric point: 4.1 ± 0.1
Michaelis constants: 2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure: One mol of FAD is covalently bound to mol of the enzyme
Inhibitors: p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH: 8.0 – 8.5
Optimum temp: 40 – 45°C
pH Stability: pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability: Below 37°C (pH 7.5, 10min)

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Assay Procedure for Choline Oxidase

One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the conditions described below.
Keywords: Atomic absorption spectroscopy, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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