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C5914 Sigma

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories Cell Culture, Cell Dissociation, Non-Enzymatic Dissociation Reagents, Reagents and Supplements
sterility   sterile-filtered
product line   BioReagent
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Can one use Product C5914, Cell Dissociation Solution, with tissue?
We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.
Does Product C5914, Cell Dissociation Solution, contain EDTA?
Yes, it does contain EDTA; the concentration is proprietary.
Does Product C5914, Cell Dissociation Solution, work with epithelial cells?
It will work with most cell types.
When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?
No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
Peer-Reviewed Papers
15

References

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M8266 Magnesium chloride, anhydrous, ≥98%
M2643 Magnesium sulfate, BioReagent, suitable for cell culture, suitable for insect cell culture
C5670 Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

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