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C5973 Sigma

Monoclonal Anti-CCR-5 antibody produced in mouse

clone 45523, purified immunoglobulin, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Chemokine Receptors, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   flow cytometry: suitable
  immunohistochemistry: 25 μg/mL using paraffin-embedded human lymph node tissue sections
clone   45523, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2b
storage temp.   −20°C
Gene Information   human ... CCR5(1234)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human CCR-5 transfected NSO mouse myeloma cells.

General description

CCR-5 is a chemokine receptor belonging to the super family of seven-transmembrane G-protein coupled receptors that mediates cellular functions of immune response, leukocyte migration, development and angiogenesis. CCR-5 is expressed on macrophages and Th1 cells activated on inflammation, osteoclasts and vascular smooth muscle cells. Three CC chemokines, CCL-3, -4 and -5 bind efficiently to CCR-5, whereas CCL-, -8 and -13 bind less efficiently. The expression of CCR-5 protein is most important in resistance to HIV-1 and hepatitis C virus infections and reportedly influences the course and progression of the infection. Monoclonal Anti-CCR-5 antibody specifically recognizes human CCR-5. The antibody shows no cross reactivity with CCR-1, CCR-2, or CCR-3.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Specificity

The antibody reacts with human CCR-5 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2 or CCR-3. This antibody can be used to detect CCR-5 present on stimulated human PBMCs that have been fixed with 2% formaldehyde, but not on unfixed cells.

Preparation Note

Purified using protein G.

Application

Monoclonal Anti-CCR-5 antibody may be used for flow cytometry; the antibody may be diluted to 25 μg/ml before adding 10 μL of this dilution to 1-2.5 X 106 cells. The antibody detects CCR-5 present on stimulated human PBMCs (peripheral blood mononuclear cells) only after the cells are fixed with 2% formaldehyde. The antibody does not detect CCR-5 on unfixed human PBMCs. For immunohistochemistry a working concentration of 25 μg/ml using paraffin-embedded human lymph node tissue sections.

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