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C5992 Sigma

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse

clone PCK-26, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-pan-Cytokeratin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   lizard, goat, bovine, human, hamster, rat, carp, guinea pig, pig, rabbit, mouse, sheep, chicken, feline, canine, snake
application(s)   dot blot: suitable
  immunocytochemistry: suitable
  immunohistochemistry: 10-20 μg/mL using human placenta or skin
  western blot: suitable
clone   PCK-26, monoclonal
concentration   ~1.5 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytokeratin from human epidermis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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F0397 Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse, clone PCK-26, purified immunoglobulin, buffered aqueous solution

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