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C6047 Sigma

Monoclonal Anti-Calponin antibody produced in mouse

clone CP-93, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers and Receptors, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells,
species reactivity   chicken, turkey, human
application(s)   electron microscopy: suitable
  immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  western blot: 1:10,000 using chicken gizzard
clone   CP-93, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 34 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CNN2(1265)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

turkey gizzard calponin

Specificity

An additional band at 29-31 kDa, representing a proteolytic fragment or the low molecular weight calponin isoform also may be stained. Using immunohistochemical staining, the antibody exhibits smooth muscle specificity. The product stains smooth muscle cells in tissue sections and primary cultures (or early passages), but not most cell lines originally derived from smooth muscle. It does not stain epithelial, endothelial or connective tissue fibroblast cells. The antibody does not cross-react with skeletal, cardiac or non-muscle tissue.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Price and Availability


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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

WGK Germany  -
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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