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C6098 Sigma

Monoclonal Anti-CCR-6 antibody produced in mouse

clone 53103.111, purified immunoglobulin, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Chemokine Receptors, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   flow cytometry: suitable using human PBMCs
  immunohistochemistry: 25 μg/mL using frozen human tissue sections
clone   53103.111, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2b
storage temp.   −20°C
Gene Information   human ... CCR6(1235)
mouse ... Ccr6(12458)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human CCR-6 transfected NSO mouse myeloma cells.

General description

Chemokine receptor CCR6 plays an important role in adaptive immunity, recruitment of CD4+ T cells and dendritic cells to sites of epithelial inflammation. It is expressed on leukocyte subsets of T cells, B cells, immature dendritic cells, neutrophils, monocytes and NKT cells. CCR6 binds the chemokine, CCL20 (macrophage inflammatory protein-3α) and induces proinflammatory signals such as TNF-α, IFN-γ and toll-like receptor agonists that in turn activate ERK1/2 and p38 MAPK pathways. CCR6 has been linked to autoimmune diseases of multiple sclerosis, rheumatoid arthritis, psoriasis and atherosclerosis. Monoclonal Anti-CCR-6 reacts with CCR-6 transfected cells and not with the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-7, CXCR-1, CXCR-2, CXCR-3, CXCR-4 and STRL33 transfected cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Specificity

The antibody reacts with human CCR-6 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-7, CXCR-1, CXCR-2, CXCR-3, CXCR-4 or STRL33.

Preparation Note

Purified using protein G.

Application

Monoclonal Anti-CCR-6 antibody may be used for flow cytometry at a working concentration of 1μg/106 cells using human peripheral blood mononuclear cells. Antibody concentration of 25 μg/ml may be used for immunohistochemistry of frozen human tissue sections.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Peer-Reviewed Papers
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References

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