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C6104 Sigma

Anti-6Ckine antibody produced in goat

affinity isolated antibody, lyophilized powder

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Properties

Related Categories 6Ckine/Exodus/SLC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Chemokines,
species reactivity   human
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... CCL21(6366)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human 6Ckine, expressed in Escherichia coli.

General description

6Ckine is a CC chemokine also known as Secondary lymphoid-tissue chemokine, Exodus-2 and thymus-derived chemotactic agent 4. It is expressed constitutively in lymphoid tissues, specifically T cell areas of lymph nodes and mucosa-associated lymphoid tissue. 6Ckine has high affinity for chemokine receptor 7 (CCR7). It acts as chemoattractant for homing T cells by allowing them to adhere to ICAM-1. Unlike other chemokines, 6Ckine is chemotactic only for lymphocytes but not monocytes. Anti-6Ckine recognizes human 6Ckine. The antibody exhibits less than 15% cross reactivity with recombinant mouse 6Ckine and less than 5% cross reactivity with recombinant human fractalkine, Teck, MCP-3, mouse CRG-2, human VIC, and mouse VIC in ELISA assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Specificity

The antibody has the ability to neutralize the biological activity of recombinant human 6Ckine. It exhibits less than 15% cross-reactivity with recombinant mouse 6Ckine and less than 5% cross-reactivity with recombinant human fractalkine, Teck, MCP-3, mouse CRG-2, and human and mouse VIC.

Application

Anti-6Ckine antibody may be used for neutralization assay; ND50 for this antibody is ~4-8 μg/ml. For immunoblotting, a working antibody concentration of 0.1-0.2 μg/ml may be used. For ELISA, a working antibody concentration of 0.5-1.0 μg/ml is recommended.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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