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C6164 Sigma

Cell Freezing Medium-DMSO 1×

sterile-filtered, suitable for cell culture



Related Categories Cell Biology, Cell Culture, Cell Freezing Media and Reagents, Culture, General Stem Cell Biology,
sterility   sterile-filtered
impurities   endotoxin, tested
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Recommended for the cryopreservation of cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

Price and Availability

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?
The DMSO is at a 10% concentration.
Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum? 
No, the serum in this cell freezing media is not heat-inactivated.
Is there insulin in Product C6164, Cell Freezing Medium-DMSO?
No, there is no added insulin in this product.  There may be traces of insulin from the serum used in this product.
Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?
Product No. C6164 is sterile-filtered.  If the sterility of the product has been compromised, it can be re-filtered.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles


Protocol for the Cryopreservation of Cell Lines

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Keywords: Cryopreservation, Phase transitions

Peer-Reviewed Papers


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