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C6165 Sigma

Carbonic Anhydrase Isozyme II human

≥80%, powder, ≥3,000 W-A units/mg protein

Synonym: Carbonate Dehydratase, Carbonate Hydrolyase

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Description

Packaging

1, 5, 25 mg in glass bottle

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Application

Human carbonic anhydrase isozyme II has been used to assess its gene fusion abilities for efficient expression and recovery of recombinant proteins. Human carbonic anhydrase isozyme II has also been used to investigate a new process for the synthesis of difluoromethanesulfonamides. Furthermore, CA II from Sigma has been used as a standard to measure the CA activity in lung tissue homogenates. The study analysed the possible relationship between the expression of carbonic anhydrase and non-small cell lung cancer. The product has also been used with Freund′s complete adjuvant at 1:1 ratio for antibody production. This study evaluated the quantitative and functional alterations of cytosolic CA isoenzymes in the erythrocytes of glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals. The enzyme has also been used in the study of natural phenolic inhibitors of CA II.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in pathological and physiological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors that can be used for treating various pathological conditions such as glaucoma, epilepsy and Parkinson’s disease. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and CO2 elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

Protocols

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 4.2.1.1) for Wilbur-Anderson Units.
Keywords: Hydration reaction, Phase transitions

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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