Synonym: Carbonate Dehydratase, Carbonate Hydrolyase
|Related Categories||4.2.x.x C-O bonds, 4.x.x.x Lyases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,|
|Gene Information||human ... CA2(760)|
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
Human carbonic anhydrase isozyme II has been used in a study to assess its gene fusion for efficient expression and rapid single-step recovery of recombinant proteins. Human carbonic anhydrase isozyme II has also been used in a study to investigate a new synthesis of difluoromethanesulfonamid
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lyophilized powder, ≥2,500 W-A units/mg protein
≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder
recombinant, expressed in E. coli, buffered aqueous solution
lyophilized powder, ≥2,000 W-A units/mg protein
Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 22.214.171.124) for Wilbur-Anderson Units.
Keywords: Hydration reaction, Phase transitions
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Gene fusions with human carbonic anhydrase II for efficient expression and rapid single-step recovery of recombinant proteins: expression of the Escherichia coli F1-ATPase epsilon subunit Van Heeke, G., et al. Protein Expr. Purif. 4, 265-74, (1993)
Speeding up proton transfer in a fast enzyme: kinetic and crystallographic studies on the effect of hydrophobic amino acid substitutions in the active site of human carbonic anhydrase II Fisher, S., et al. Biochemistry 46, 3803-13, (2007)
Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression: a Gynaecological Oncology Group Study. Liao SY, Rodgers WH, Kauderer J, et al. Br. J. Cancer 108(3), 613-20, (2013)
Elevated CO2 levels affect the activity of nitrate reductase and carbonic anhydrase in the calcifying rhodophyte Corallina officinalis. Hofmann LC, Straub S, and Bischof K J. Exp. Bot. 64(4), 899-908, (2013)
Hypoxia inducible factor-1 alpha and carbonic anhydrase IX overexpression are associated with poor survival in breast cancer patients. Kaya AO, Gunel N, Benekli M, et al. J. BUON. 17(4), 663-8, (2012)
Lowering of elevated tissue PCO2 in a hemorrhagic shock rat model after reinfusion of a novel nanobiotechnological polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase that is an oxygen and a carbon dioxide carrier with enhanced antioxidant properties. Bian Y, Wei G, and Chang TM Artif. Cells. Nanomed. Biotechnol. 41(1), 60-8, (2013)
Pharmacological inhibition of carbonic anhydrase XII interferes with cell proliferation and induces cell apoptosis in T-cell lymphomas. Lounnas N, Rosilio C, Nebout M, et al. Cancer Lett. 333(1), 76-88, (2013)
Peroxisome proliferator activated receptor-α/hypoxia inducible factor-1α interplay sustains carbonic anhydrase IX and apoliprotein E expression in breast cancer stem cells. Papi A, Storci G, Guarnieri T, et al. PLoS ONE 8(1), e54968, (2013)
Dendritic cell-based immunotherapy in prevention and treatment of renal cell carcinoma: efficacy, safety, and activity of Ad-GM·CAIX in immunocompetent mouse models. Birkhäuser FD, Koya RC, Neufeld C, et al. J. Immunother. 36(2), 102-11, (2013)
Evaluation of morphologically unclassified renal cell carcinoma with electron microscopy and novel renal markers: implications for tumor reclassification. Talento R, Hewan-Lowe K, and Yin M Ultrastruct. Pathol. 37(1), 70-6, (2013)
Crystallization, characterization and preliminary X-ray crystallographic analysis of GK2848, a putative carbonic anhydrase of Geobacillus kaustophilus. Ragunathan P, Raghunath G, Kuramitsu S, et al. Acta Crystallogr. F, Struct. Biol. Cryst. Commun. 69(Pt 2), 162-4, (2013)
Targeted mutagenesis of mitochondrial carbonic anhydrases VA and VB implicates both enzymes in ammonia detoxification and glucose metabolism. Shah GN, Rubbelke TS, Hendin J, et al. Proc. Natl. Acad. Sci. U. S. A. 110(18), 7423-8, (2013)
The usefulness of three-dimensional cell culture in induction of cancer stem cells from esophageal squamous cell carcinoma cell lines. Fujiwara D, Kato K, Nohara S, et al. Biochem. Biophys. Res. Commun. 434(4), 773-8, (2013)
4-Substituted-2,3,5,6-tetrafluorobenzenesulfonamides as inhibitors of carbonic anhydrases I, II, VII, XII, and XIII. Dudutienė V, Zubrienė A, Smirnov A, et al. Bioorg. Med. Chem. 21(7), 2093-106, (2013)
Carbonic anhydrase IX (CAIX) is not an independent predictor of outcome in patients with clear cell renal cell carcinoma (ccRCC) after long-term follow-up. Zhang BY, Thompson RH, Lohse CM, et al. BJU Int. 111(7), 1046-53, (2013)
Carbonic anhydrase type IX expression in lobular endocervical glandular hyperplasia and gastric-type adenocarcinoma of the uterine cervix. Mikami Y, Minamiguchi S, Teramoto N, et al. Pathol. Res. Pract. 209(3), 173-8, (2013)
Cloning, characterization, and sulfonamide and thiol inhibition studies of an α-carbonic anhydrase from Trypanosoma cruzi, the causative agent of Chagas disease. Pan P, Vermelho AB, Capaci Rodrigues G, et al. J. Med. Chem. 56(4), 1761-71, (2013)
Clear cell papillary renal cell carcinoma-like tumors in patients with von Hippel-Lindau disease are unrelated to sporadic clear cell papillary renal cell carcinoma. Williamson SR, Zhang S, Eble JN, et al. Am. J. Surg. Pathol. 37(8), 1131-9, (2013)
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