• USA Home
  • C6170 - Concanavalin A from Canavalia ensiformis (Jack bean)

EMAIL THIS PAGE TO A FRIEND
C6170 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

agarose conjugate, Type V-B, buffered aqueous suspension

Synonym: Con A

Purchase

Properties

type   Type V-B
form   buffered aqueous suspension
extent of labeling   ~15 mg per mL
matrix   4% beaded agarose
matrix activation   p-nitrophenyl chloroformate
matrix spacer   1 atom
capacity   ~6 mg/mL binding capacity (yeast mannan)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Physical form

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, 1 mM each Mn2+, Mg2+, and Ca2+, and 0.02% thimerosal

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?