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  • C6198 - Anti-Actin, α-Smooth Muscle - Cy3 antibody, Mouse monoclonal

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C6198 Sigma

Anti-Actin, α-Smooth Muscle - Cy3 antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

Synonym: Monoclonal Anti-Actin, α-Smooth Muscle, SMA

Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology,
species reactivity   rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix
clone   1A4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~42 kDa
shipped in   wet ice
storage condition   protect from light
storage temp.   2-8°C
Gene Information   human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
biological source   mouse
conjugate   CY3 conjugate
Featured Industry   Research Pathology

Description

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

General description

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Application

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of GE Healthcare

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