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C6219 Sigma

Anti-Connexin-43 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology,
species reactivity   mammals, human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using human or animal tissue sections
  immunohistochemistry (frozen sections): 1:2,000 using human or animal tissue sections
  indirect immunofluorescence: 1:400 using cultured BHK cells
  microarray: suitable
  western blot: 1:8,000 using an animal tissue extract
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GJA1(2697)
mouse ... Gja1(14609)
rat ... Gja1(24392)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal segment of the cytoplasmic domain (amino acids 363-382 with N-terminally added lysine) of human/rat connexin-43.

General description

Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights ranging from 26 to 70 kDa. At least a dozen distinct connexin genes have been identified in mammals, many expressed in a diverse tissue and cell specific pattern. Two distinct lineages have been identified in mammals, one termed class I or β group, in which Cx26, Cx30, Cx31, Cx31.1 and Cx32 fall, and the other termed class II or α group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46.
The 43 kDa connexin protein (connexin 43, Cx43) is a phosphoprotein that is expressed in most tissues, even though the pattern of expression may differ in various cell types (e.g., in the brain, it is found in astrocytes, ependyma and leptomeninges, but not in neurons, oligodendrocytes and pinealocytes; or in the liver, it is present in Ito cells, but not in hepatocytes). Gap junction protein levels change in response to disruption of tissue architecture. For instance, an increased expression of Cx43 was found in early stages of atherosclerosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Anti-Connexin 43 reacts specifically with connexin 43. By immunoblotting, the antibody detects a single band or 2-3 bands at 43 kDa region. Staining of connexin 43 band(s) by immunoblotting is specifically inhibited with the connexin 43 peptide (amino acid residues 363-382). Reactivity has been observed with human, bovine, rat, mouse, hamster and chicken connexin 43.

Application

Anti-Connexin-43 may be used in immunoblotting, immunocytochemistry and immunohistochemistry (frozen and formalin-fixed, paraffin-embedded tissues). Polyclonal antibodies reacting specifically with Cx43 may be applied in diverse cellular and molecular approaches to the study of gap junctions and their properties.
A minimum working dilution of 1:8,000 is determined by immunoblotting using a whole extract from mouse brain.
A minimum working dilution of 1:400 is determined by indirect immunofluorescent staining of acetone-fixed cultured baby hamster kidney (BHK).
A minimum working dilution of 1:2,000 is determined by indirect immunofluorescent staining of rat heart. (Negative on rat liver sections)
A minimum working dilution of 1:2,000 is determined by indirect immunoperoxidase staining of trypsin-digested,formalin-fixed, paraffin-embedded human or animal tissue.

Price and Availability


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