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C6223 Sigma

Monoclonal Anti-CXCR-1 (IL-8 RA) antibody produced in mouse

clone 42705.111, purified immunoglobulin, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Chemokine Receptors, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   flow cytometry: 10 μg/mL using using human blood cells (10 μL diluted antibody/105-106 cells)
  immunohistochemistry (frozen sections): 25 μg/mL using human tissue (speen)
  neutralization: suitable
clone   42705.111, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2a
storage temp.   −20°C
Gene Information   human ... IL8RA(3577)
mouse ... Il8ra(227288)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human CXCR1 transfected NSO mouse myeloma cells.

General description

Chemokine receptor CXCR1 also refers as type I IL-8 receptor or IL-8RA is a class A, rhodopsin-like G-protein-coupled receptor, expressed mainly in neutrophils but not in T or B lymphocytes . It has a crucial role in cellular signal transduction and targeted as drug receptors. Monoclonal anti-CXCR-1 (IL-8 RA) antibody can be used to neutralize the bioactivity facilitated by human cell surface CXCR-1. Mouse anti-CXCR-1 (IL-8 RA) antibody reacts specifically with CXCR-1 transfected cells but not with the parent cell line. The product has also shown no cross-reactivity with human CXCR-2.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Specificity

The antiboody, in the presence of anti-CXCR2 (Sigma Product No. C6348), will neutralize human cell surface CXCR-1 mediated-myloperoxidase release from human granulocytes induced by IL-8. The antibody reacts with human CXCR1 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CXCR2.

Preparation Note

Purified using protein G.

Application

Monoclonal anti-CXCR-1 (IL-8 RA) antibody can be used in flow cytometry for detecting CXCR-1 present on human blood cells. It can also be used in immunohistochemistry (25 μg/mL) using frozen human tissue sections (spleen).

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