• USA Home
  • C6361 - Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit

C6361 Sigma

Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit

~1 mg/mL, buffered aqueous glycerol solution, affinity isolated antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   human
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot (chemiluminescent): 1:5,000
  western blot: 1:1,000
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CAPN3(825)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to insert 1 in domain-II of the large subunit of human calpain-94 (calpain-3, nCANP, nCL-1, calpain p94, muscle calpain).

Physical form

Solution in 0.01 M phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.


Recognizes latent and active calpain-94. Binds the reduced and non-reduced protein. Identifies bands at 94 kDa, 82 kDa, 62 kDa, 60 kDa, and a series of further cleaved active forms. The rat 88 kDa and 90 kDa calpains also contain insert 1, so the antibody may cross-react with these forms.


Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit is suitable for the following applications:
• Immunohistochemistry (frozen sections)
• Immunoprecipitation
• Indirect ELISA
• Western blot (chemiluminescent) at a dilution of 1:5,000
• Western blotting at a dilution of 1:1,000

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Calpain is a heterodimer consisting of a larger and a smaller subunit. It is a major intracellular protease. Calpains are a family of calcium-dependent thiol-proteases, which are involved in many physiological processes and pathological conditions. These are calcium-activated non-lysosomal thiolproteases. m- and μ-calpain are ubiquitously expressed and are countered by the endogenous calpain inhibitor calpastatin. Calpains are present in all mammalian tissues and are involved in a variety of processes including cell proliferation, differentiation, vesicle secretion and others. It is also likely to be involved in processing of numerous enzymes and cytoskeletal components by linking their activity to a variety of intracellular events.

Price and Availability

Antibody Explorer

Explore with Confidence

Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany  1
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?