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C6361 Sigma

Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit

~1 mg/mL, buffered aqueous glycerol solution, affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   human
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot (chemiluminescent): 1:5,000
  western blot: 1:1,000
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CAPN3(825)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to insert 1 in domain-II of the large subunit of human calpain-94 (calpain-3, nCANP, nCL-1, calpain p94, muscle calpain).

Physical form

Solution in 0.01 M phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.

Specificity

Recognizes latent and active calpain-94. Binds the reduced and non-reduced protein. Identifies bands at 94 kDa, 82 kDa, 62 kDa, 60 kDa, and a series of further cleaved active forms. The rat 88 kDa and 90 kDa calpains also contain insert 1, so the antibody may cross-react with these forms.

Application

Anti-Calpain-94 (Domain II, Insert 1), Large Subunit antibody produced in rabbit is suitable for the following applications:
• Immunohistochemistry (frozen sections)
• Immunoprecipitation
• Indirect ELISA
• Western blot (chemiluminescent) at a dilution of 1:5,000
• Western blotting at a dilution of 1:1,000

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Calpain is a heterodimer consisting of a larger and a smaller subunit. It is a major intracellular protease. Calpains are a family of calcium-dependent thiol-proteases, which are involved in many physiological processes and pathological conditions. These are calcium-activated non-lysosomal thiolproteases. m- and μ-calpain are ubiquitously expressed and are countered by the endogenous calpain inhibitor calpastatin. Calpains are present in all mammalian tissues and are involved in a variety of processes including cell proliferation, differentiation, vesicle secretion and others. It is also likely to be involved in processing of numerous enzymes and cytoskeletal components by linking their activity to a variety of intracellular events.

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Safety Information

WGK Germany  1
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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