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C6638 Sigma

Creatine Phosphokinase from rabbit brain

Type IV, lyophilized powder, 50-150 units/mg protein

Synonym: ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase

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Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Phosphorylates ADP to form ATP using phosphocreatine as the phosphate donor.

Caution

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Unit Definition

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

Physical form

Lyophilized powder containing buffer salt as glycine, pH 9.0.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

HPLC Analysis of Creatine Kinase on Discovery® BIO PolyMA-WAX

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Creatine Kinase on Discovery® BIO PolyMA-WAX
Keywords: Chromatography, High performance liquid chromatography

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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