|Related Categories||Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,|
Contains 30 mg substrate per tablet.
Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.
4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.
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ready to use solution
for analytical purposes, ≥99.0%
Certificate of Analysis
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26-36|
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
Nakane, P.K. J. Histochem. Cytochem. 6, 557, (1968)
Gap junctions in the ovary of Drosophila melanogaster: localization of innexins 1, 2, 3 and 4 and evidence for intercellular communication via innexin-2 containing channels. Bohrmann J BMC Dev. Biol. 8, 111, (2008)
Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China. Chen N, Tong C, Li D, et al. Virol. J. 7, 378, (2010)
Imaging of DNA hybridization on microscopic polypyrrole patterns using scanning electrochemical microscopy (SECM): the HRP bio-catalyzed oxidation of 4-chloro-1-naphthol. Fortin E, Mailley P, Lacroix L, et al. Analyst 131(2), 186-93, (2006)
Visualization of antigen on nitrocellulose membrane by the oxidative coupling reaction of N,N'-dimethyl-p-phenylenediamine and 4-chloro-1-naphthol. Kobayashi R and Tashima Y Anal. Biochem. 183(1), 9-12, (1989)
Fully automated cytochemistry on whole blood smears using the Technicon Hemalog D or Technicon H6000/Autoslide: myeloperoxidase staining methods. Lippi U, Cappelletti P, and Schinella M Nouv. Rev. Fr. Hematol. 29(5), 321-5, (1987)
Aldrich MSDS 1, 425:D / Corp MSDS 1, 815:C / Corp MSDS 1 (1), 815:C / FT-IR 2 (2), 1895:B / FT-IR 1 (1), 1109:D / FT-NMR 1 (2), 308:B / IR-Spectra (3), 667:G / IR-Spectra (2), 596:A / NMR-Reference 2 (1), 909:B / RegBook 1 (1), 1303:D / Sigma FT-IR 1 (2), 384:A / Structure Index 1, 199:B:1
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