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C7104 Sigma

Monoclonal Anti-Cdc27 antibody produced in mouse

~2 mg/mL, clone AF3.1, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cyclins and CDKs,
species reactivity   rat, human, bovine, Xenopus, mouse, canine
application(s)   immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using a HeLa Cell nuclear extract
clone   AF3.1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
mol wt   antigen mol wt 97 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

General description

The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
• Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
• Immunoprecipitation.
• Indirect ELISA
• Microarray
• Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
• Anaphase-promoting complex/cyclosome APC/C Purification.

Biochem/physiol Actions

CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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