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C7275 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

Type V, lyophilized powder

Synonym: Con A

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Properties

Related Categories Concanavalin A (Con A), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
type   Type V
form   lyophilized powder
potency   ≤64 μg per mL agglutination activity
impurities   Carbohydrate, essentially free
  salt, essentially free
  ≤0.16 wt. % Ca
  ≤0.23 wt. % Mn
storage temp.   −20°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Preparation Note

Produced from whole Jack beans (J0250) using the method of Sophianopoulos.

Quality

Highly purified.

Price and Availability


Laboratory Gloves

Amplified Detection
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
GK6890000
Protocols & Articles
Peer-Reviewed Papers
15

References

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