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C7354 Sigma

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies for Neural Stem Cells,
species reactivity   chicken, rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections
  microarray: suitable
  western blot: 1:1,000 using recombinant rat calbindin-D-28K
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 28 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CALB1(793)
mouse ... Calb1(12307)
rat ... Calb1(83839)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit is suitable for the following applications:
• Immunohistochemistry ((formalin-fixed, paraffin-embedded sections) at a dilution of 1:500 using sections of rat, human or chicken cerebellum sections)
• Microarray
• Western blotting at a dilution of 1:1,000 using recombinant rat calbindin-D-28K

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Analysis Note

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining.

Biochem/physiol Actions

Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.

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Safety & Documentation

Safety Information

Symbol  GHS07  GHS07
Signal word  Warning
Hazard statements  H302-H412
Precautionary statements  P273-P301 + P312 + P330
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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