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C7372 Sigma

Anti-CLGN (496-510) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Calmegin precursor



Related Categories Alphabetical Index, Antibodies, CI-CN, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~70 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CLGN(1047)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 496-510 of human CLGN

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. Anti-CLGN (496-510) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000.

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Biochem/physiol Actions

Calmegin (CLG) is a protein encoded by the CLGN gene in humans. Human Calmegin is mapped to chromosome 4q28.3-q31.1. It is a testis-specific molecular chaperon that plays a key role in spermatogenesis. Calmegin (Ca2+-binding protein) is known to be a homologue of endoplasmic reticulum (ER) that resides on lectin chaperone calnexin. It is transcriptionally regulated by histone deacetylase (HDAC) and CpG methyltransferase. Calmegin is useful as a maker molecule to study the functional role of the endoplasmic reticulum in the process of spermatid differentiation.

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