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C7423 Sigma

Monoclonal Anti-CD8 antibody produced in mouse

clone UCHT-4, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD8



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
clone   UCHT-4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD8A(925), CD8B(926)
biological source   mouse
conjugate   unconjugated



human thymocytes followed by peripheral blood T cells.

Antigen Background

CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)

Monoclonal Anti-CD8 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers


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