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C7482 Sigma

Caspase 6 human

recombinant, expressed in E. coli, >90% (SDS-PAGE), lyophilized powder, 13,000 units/mg protein

Synonym: Mch2

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Properties

recombinant   expressed in E. coli
assay   >90% (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt ~30 kDa
solubility   PBS: soluble
shipped in   dry ice
storage temp.   −70°C

Description

Biochem/physiol Actions

Cleaves the substrates consisting of consensus sequence VEID.

Spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme.

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays.

Unit Definition

One unit will hydrolyze 1 nmol of the caspase substrate VEID-pNA to VEID and p-nitroaniline per hour at pH 7.2 at 37 °C.

Physical form

Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
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Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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