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C7492 Sigma

Monoclonal Anti-Calreticulin antibody produced in mouse

clone TO-11, tissue culture supernatant, buffered aqueous solution

Synonym: Anti-Autoantigen Ro

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Properties

Related Categories AA - CA, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling,
species reactivity   human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: 1:50-1:100 using total cell extract of HeLa cells
clone   TO-11, monoclonal
antibody form   tissue culture supernatant
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CALR(811)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 383-399 of human calreticulin, conjugated to KLH.

Physical form

Culture supernatant solution containing 15 mM sodium azide.

Application

Monoclonal Anti-Calreticulin antibody produced in mouse is suitable for the following applications:
• Flow cytometry
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Western blotting at a dilution of 1:50-1:100 using total cell extract of HeLa cells

Biochem/physiol Actions

Calreticulin is a protein binding Ca2+ ions. It is encoded by the CALR gene in humans. Its variants causes differential effects on thrombopoiesis. Somatic mutations of calreticulin (CALR) gene causes thrombocythemia (ET) and primary myelofibrosis (PMF).

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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