Concanavalin A from Canavalia ensiformis (Jack bean) agarose conjugate, Type VI, saline suspension

Properties

Related Categories	Affinity Chromatography, Bioactive Small Molecules, CI-CY, Carbohydrate Binding Matrices, Cell Biology, Glycobiology, Glycoprotein Purification, Lectin Matrices, Post-Translational Modification, Protein Chromatography, Proteomics More...
type	Type VI
form	saline suspension
extent of labeling	15-30 mg concanavalin A protein per mL
matrix	cross-linked 4% beaded agarose
matrix activation	cyanogen bromide
matrix attachment	amino
matrix spacer	1 atom
capacity	5-15 mg/mL binding capacity (yeast mannan)(in 0.01 M phosphate buffered saline, pH 6.8, containing 1 mM each CaCl₂, MgCl₂ and MnCl₂)
conjugate	agarose conjugate
storage temp.	2-8°C

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Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Used for the purification of glycoproteins containing mannose

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.