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C7555 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

agarose conjugate, Type VI, saline suspension

Synonym: ConA

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Properties

Related Categories Affinity Chromatography, Carbohydrate Binding Matrices, Glycobiology, Glycoprotein Purification, Lectin Matrices,
type   Type VI
form   saline suspension
extent of labeling   15-30 mg concanavalin A protein per mL
matrix   cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   5-15 mg/mL binding capacity (yeast mannan)(in 0.01 M phosphate buffered saline, pH 6.8, containing 1 mM each CaCl2, MgCl2 and MnCl2)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Used for the purification of glycoproteins containing mannose

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Physical form

Suspension in 1.0 M NaCl + 0.1 M potassium phosphate, pH 6.0, with 1 mM CaCl2, 1 mM MgCl2 and 1 mM MnCl2 containing 0.02% thimerosal

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

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