|Related Categories||Affinity Chromatography, Bioactive Small Molecules, CI-CY, Carbohydrate Binding Matrices, Cell Biology,|
|extent of labeling||15-30 mg concanavalin A protein per mL|
|matrix||cross-linked 4% beaded agarose|
|matrix activation||cyanogen bromide|
|matrix spacer||1 atom|
|capacity||5-15 mg/mL binding capacity (yeast mannan)(in 0.01 M phosphate buffered saline, pH 6.8, containing 1 mM each CaCl2, MgCl2 and MnCl2)|
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Used for the purification of glycoproteins containing mannose
Con A is not blood group specific but has an affinity for terminal α-
Suspension in 1.0 M NaCl + 0.1 M potassium phosphate, pH 6.0, with 1 mM CaCl2, 1 mM MgCl2 and 1 mM MnCl2 containing 0.02% thimerosal
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Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins. Lotan, R Biochemistry 16, 1787-1794, (1977)
None, Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
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