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C7559 Sigma

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

Synonym: Methylidyne trichloride, Trichloromethane

  • CAS Number 67-66-3

  • Empirical Formula (Hill Notation) CHCl3

  • Molecular Weight 119.38

  •  Beilstein Registry Number 1731042

  •  EC Number 200-663-8

  •  MDL number MFCD00000826

  •  PubChem Substance ID 24892970

  • Popular Documents:  FTNMR (PDF)  

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Properties

Related Categories Chloroform, Solvent by Type, Solvents More...
grade   PCR Reagent
vapor density   4.1 (vs air)
vapor pressure   160 mmHg ( 20 °C)
InChI Key   HEDRZPFGACZZDS-UHFFFAOYSA-N
assay   ≥99%
contains   amylenes as stabilizer
packaging   vial of 1.5 mL
color   colorless
refractive index   n20/D 1.445(lit.)
bp   60.5-61.5 °C(lit.)
mp   −63 °C(lit.)
density   1.492 g/mL at 25 °C(lit.)
foreign activity   DNase, RNase, none detected

Description

Application

Facilitates recovery of the aqueous phase of PCRs which have been overlaid with mineral oil.

Packaging

5 vials in serum bottle

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Precautionary statements 
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
3
RTECS 
FS9100000
Protocols & Articles

Protocols

Amplification of DNA Using Jumpstart REDTaq DNA Polymerase D8187 Protocol

Note: The use of DMSO or formamide with JumpStart REDTaq DNA Polymerase is not recommended due to interference with the enzyme-antibody complex. Other co-solvents, solutes (e.g., salts) and extremes ...
Keywords: Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Amplification of DNA Using Jumpstart Taq DNA Polymerase D9307 Protocol

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...

Reduce Non-specific PCR Amplification- Jumpstart Taq Polymerase D4148 Protocol

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Peer-Reviewed Papers
15

References

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