• USA Home
  • C7594 - Casein Blocking Buffer

EMAIL THIS PAGE TO A FRIEND
C7594 Sigma

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Purchase

Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents More...
grade   for molecular biology
form   powder blend
pH range   6.8 - 7.2 (reconstituted per directions)
solubility   H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
storage temp.   room temp

Description

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Features and Benefits

• Better blocking performance than dried milk or BSA
• Compatible with neutral and positively-charged membranes
• Compatible with chemiluminescent, colorimetric , and DIG detection assays

General description

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Packaging

1 L in poly bottle

Preparation Note

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Protocols

Casein Blocking Buffer for Direct Detection Southern or Western Blotting

Reagents Required but not Provided Preparation Storage/Stability Procedure Results Materials References
Keywords: Detergents, Enzyme activity, Immobilization, Mass spectrometry, PAGE, Southern blot, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

P7059 Phosphate Buffered Saline, 10× PBS for Western blots and IP
B6429 Casein Blocking Buffer 10x, for Northern and Southern blotting, solution
H7140 Hybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)
M5755 MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers
H7033 PerfectHyb Plus Hybridization Buffer, for Northern and Southern blotting, solution
D9905 Denhardt’s Solution, for Northern and Southern blotting, lyophilized powder
D2532 Denhardt’s Solution 50x, for Northern and Southern blotting, solution
P1415 Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)
85639 SSC Buffer, for Northern and Southern blotting, powder blend
93017 SSC Buffer 20x, for Northern and Southern blotting, solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?