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  • C7604 - Anti-γ-Tubulin-Cy3 antibody produced in rabbit

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C7604 Sigma

Anti-γ-Tubulin-Cy3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-γ-Tubulin

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   chicken, human
application(s)   direct immunofluorescence: 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 48 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... TUBG1(7283), TUBG2(27175)
biological source   rabbit
conjugate   CY3 conjugate

Description

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

General description

Tubulin, γ 1 is a protein encoded by the TUBG1 gene in humans. This gene encodes a member of the tubulin superfamily. γ-tubulin interacts with Arp2/3, a complex that nucleates actin filaments and with the Arp2/3 activator WASH. It is found to have relationship with actin filament dynamics.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

Anti-γ-Tubulin-Cy3 antibody produced in rabbit is suitable for direct immunofluorescence at a dilution of 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of GE Healthcare

GraviTrap is a trademark of GE Healthcare

Biochem/physiol Actions

γ-Tubulins in the γ-TuRC might promote microtubule nucleation by providing a template that enhances the intrinsically weak lateral interaction between αβ-tubulin heterodimers when associated laterally. It has a unique functional property of a monomeric tubulin. γ-tubulin is also found to be expressed in lung cancer. Cep70 as an important centrosomal protein that interacts with γ-tubulin and plays a critical role in the regulation of mitotic spindle assembly.

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