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C7611 Sigma

Monoclonal Anti-Caspase 12 antibody produced in rat

clone 14F7, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
species reactivity   rat, mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells
clone   14F7, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Casp12(12364)
rat ... Casp12(156117)
biological source   rat
conjugate   unconjugated

Description

Immunogen

recombinant mouse caspase 12 (residues 95-318).

General description

Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Recognizes full length and possibly truncated p20 caspase 12.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Caspase 12 antibody produced in rat is suitable for the following applications:
• Immunocytochemistry
• Immunohistochemistry
• Immunoprecipitation
• Microarray
• Western blotting at a concentration of 1-2μg/mL using whole cell extract of rat kidney cells NRK cells
It is used for Immunoblotting at a dilution of 1:200.

Biochem/physiol Actions

Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.

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Safety Information

RIDADR  UN 3082 9 / PGIII
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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