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C7617 Sigma

Anti-Calnexin antibody, Mouse monoclonal

clone TO-5, purified from hybridoma cell culture

Synonym: Anti-CANX, Anti-CNX

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.
clone   TO-5, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~90 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CANX(821)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 509-524 of human calnexin, conjugated to KLH through a N-terminal added cysteine.

General description

Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca2+-dependent retention of proteins.

Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Mouse monoclonal clone TO-5 anti-Calnexin antibody is useful in ELISA, immunoblotting, flow cytometry, immunocytochemistry and immunohistochemistry.

Price and Availability


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